Specification :

Concentration: 75ng/ µ l

Recommended Loading

2-5 µ l/ Lane.

Concentration

Typical Bands 125 ng/ 5µ l

Other Bands 50 ng/ 5µ l

Recommended Electrophoresis Condition

5 µ l/ Lane, 8 cm 1 % Agarose Gel, 1× TAE, 7 V/ cm, 45min

Contents( bp)

100, 300, 500, 700, 900, 1, 200

Storage

1 year at room temperature, for long-time preservation, please storage at -20° C .

Description

Marker 2 is ideal for determining the size of double-stranded DNA from 200 to 4500 base pairs. The ladder consists of 6 linear double-stranded fragments .The 700bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ul loading, all fragments except 1200bp are 50ng. The 1200bp fragment is 125ng. This ladder is pre-mixed with loading dye and is ready to use.

Recommendations

Do not heat before loading.

For quantification, adjust the concentration of the sample to equalize

it approximately with the amount of DNA in the nearest band of the ladder.

Visualize DNA by staining with ethidium bromide or with SYBR® Green I.