custom antibody services

Price:

please inquire

Quantity Order:

Origin:

China

Payment Method:

Telegraphic Transfer (T/T)

Pack. & Delivery:

at your requirement

1. Polyclonal Antibody

GLS is able to offer custom antibody production using our 70-days program.

Polyclonal Antibody Program:

70-day conventional program or NIH 90-day program

pre-immune serum (As a negative control serum): 1x 2ml

anti-serum: 2x 30 ml

Additional Services:

ELISA

Custom Peptide Synthesis

Quantity: 10-14mg

Purity: >85%

Certificate of Analysis, HPLC report & MS data

Conjugation

KLH

BSA

Complete Anti-Peptide Antibody Package includes:

Selection of epitope

Custom peptide synthesis: 5 mg purified (up to 15 mer amino acid residues, >85% purity)

BSA conjugation (ELISA screening): 1 mg

KLH conjugation (antibody production): 4mg

Immunization as required

Peptide-specific ELISA titration of antibody for all bleeds

Pre-immune samples of serum, ascites, or eggs

Delivery to customer:100ml anti-serum, 20ml ascites, or 12antibody-containing eggs

2. Monoclonal Antibody

GLS provides the following hybridoma development program:

Timeline for completion in 5-6 months

Tailored to the customer need by providing services on the selection of epitope

Evaluation of suitable screening methods

All information is will be kept under ‘Confidential’

Development is setup in steps. Billing is at the beginning of each step, and customer will not be required to pay if the next step is not executed.

Monoclonal Antibody Production Program:

Step 1: Immunization (30 days)

Step 2: Fusion (21 days)

Step 3: Monoclonal Selection and Preservation (21 days)

Step 4 : Antibody Production (21 days)

3. Conjugation Services

GLS offers the following conjugation services at a very competitive price.

KLH conjugation

BSA conjugation

A quality report will be provided on the confirmation of conjugation

The efficiency of conjugation is above 85%

4. Phosphospecific Antibody

GLS can provide phosphospecific antibody which includes:

phosphopeptide synthesis –15 mg purified (>90%, up to 15 mer amino acid residues)

phosphospecific epitope affinity purification: 5mg

KLH conjugation used for antibody production: 4mg

BSA conjugation: 1mg

Delivery to customer: 5mg

Non-phosphopeptide synthesis –11 mg purified (>80%, up to 15 mer amino acid residues)

Affinity depletion: 5mg

BSA conjugation: 1mg

Delivery to customer : 5mg

Immunization : 2 rabbits

Phosphopeptide and non-phosphopeptide ELISA titration of antibody for all bleeds

Pre-immune sample of serum (5 mL/each rabbit) and anti-serum (100 mL total)

Affinity purification of serum

Depletion of nonphospecific peptide matrix

Shipment of purified phosphospecific antibody (confirmed by ELISA)

5. Antibody Purification and Labeling

GLS offers the following antibody purification

ELISA titering of peptide-antibody

Epitope affinity purification

Preparation of affinity matrix

Protein A/G purification

SDS-PAGE analysis of antibody

GLS offers the following antibody labeling services:

Horseradish Peroxidase (HRP)

Origin: China
Price: please inquire
Payment Method: Telegraphic Transfer (T/T), Western Union
Pack. & Delivery: at your requirement

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