Protein A Sepharose" CL-4B

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Origin:

China

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Telegraphic Transfer (T/T)

Protein A Sepharose" CL-4B is protein A immobilized by the CNBr method to Sepharose CL-4B.
Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen. Hence, protein A Sepharose CL-4B is extremely useful for isolating of immune complexes.
Technical Information
TECHNICAL SPECIFICATIONS
Ligand Native Protein A
Ligand coupling method Cyanogen bromide activation
Matrix Cross-linked agarose, 4%
Average particle size 90 µm
Binding capacity » 20 mg human IgG/ml medium
Ligand density » 3 mg Protein A/ml medium
pH stability 3-9 (long term), 2*-10 (short term)
Storage (swollen medium) 20% ethanol
Storage temperature 4¡ãC to 8¡ãC
* pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

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